Unit

UNIT 4.3 Expression Cloning of Neural Genes Using Xenopus laevis Oocytes

  1. Jim Boulter1,
  2. Christopher Boyer2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.NS0403s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Boulter, J. and Boyer, C. 2001. Expression Cloning of Neural Genes Using Xenopus laevis Oocytes. Current Protocols in Neuroscience. 00:4.3:4.3.1–4.3.24.

Author Information

  1. 1

    University of California at Los Angeles, Los Angeles, California

  2. 2

    The Salk Institute, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997

Abstract

Expression cloning requires a representative cDNA or genomic DNA library and a host organism in which the cloned genes can be transcribed and/or translated. It likewise requires a method to detect the expressed protein using, for example, the inherent biological activity of the gene or antibodies specific for the gene product. Most successful expression cloning strategies have employed cDNA libraries constructed in plasmid or bacteriophage lambda vectors and Xenopus oocytes or cultured mammalian cells as hosts. This unit presents several protocols designed for expression cloning paradigms that rely on electrophysiological recordings from Xenopus laevis oocytes.