Unit

UNIT 1.3 Hybridization Histochemistry of Neural Transcripts

  1. W. Scott Young III,
  2. Éva Mezey

Published Online: 1 FEB 2004

DOI: 10.1002/0471142301.ns0103s25

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Young, W. S. and Mezey, É. 2004. Hybridization Histochemistry of Neural Transcripts. Current Protocols in Neuroscience. 25:1.3:1.3.1–1.3.20.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: OCT 2003

Abstract

Expression of genes is manifested by the production of RNA transcripts within cells. Hybridization histochemistry (or in situ hybridization) permits localization of these transcripts with cellular resolution or better. Furthermore, the relative amounts of transcripts detected within different tissues or the same tissues in different states (e.g., physiological or developmental) may be quantified. This unit describes hybridization histochemical techniques using either oligonucleotide probes or RNA probes (riboprobes). Also presented is the use of probes labeled with digoxigenin for colorimetric detection of RNA transcripts and a technique to detect the Y chromosome using either mouse or human riboprobes. Finally, a procedure is presented for the autoradiographic detection of radiolabeled probes. Methods are provided for labeling oligodeoxynucleotide and RNA probes and performing northern analyses using these probes.