Unit

UNIT 1.4 Receptor Binding Techniques

  1. Kirk A. Frey1,
  2. Roger L. Albin2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0104s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Frey, K. A. and Albin, R. L. 2001. Receptor Binding Techniques. Current Protocols in Neuroscience. 00:1.4:1.4.1–1.4.14.

Author Information

  1. 1

    University of Michigan, Ann Arbor, Michigan

  2. 2

    University of Michigan and Ann Arbor Veterans' Administration Medical Center, Ann Arbor, Michigan

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997

Abstract

This overview first discusses issues relating to the selection of radioligand for receptor binding assays, including the isotopic label and considerations pertaining to the pharmacological and chemical profile of the ligand. This is followed by a section on characterization of ligand-binding assays, starting with tissue preparation methods, followed by detection of specific binding, determination of incubation and washing conditions and a discussion of saturation and competition assay formats. Quantification of the assay results can be accomplished by autoradiography or film densitometry. Finally, methods and considerations for analysis of the resulting data are presented.