UNIT 1.8 Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH)
Published Online: 1 AUG 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Guzowski, J. F. and Worley, P. F. 2001. Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH). Current Protocols in Neuroscience. 15:1.8:1.8.1–1.8.16.
- Published Online: 1 AUG 2001
- Published Print: MAY 2001
This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.