Unit

UNIT 1.8 Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH)

  1. John F. Guzowski1,
  2. Paul F. Worley2

Published Online: 1 AUG 2001

DOI: 10.1002/0471142301.ns0108s15

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Guzowski, J. F. and Worley, P. F. 2001. Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH). Current Protocols in Neuroscience. 15:1.8:1.8.1–1.8.16.

Author Information

  1. 1

    University of Arizona, Tucson, Arizona

  2. 2

    Johns Hopkins University, School of Medicine, Baltimore, Maryland

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: MAY 2001

Abstract

This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.