UNIT 1.14 Anterograde Axonal Tract Tracing
Published Online: 1 NOV 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Raju, D. V. and Smith, Y. 2006. Anterograde Axonal Tract Tracing. Current Protocols in Neuroscience. 37:1.14:1.14.1–1.14.11.
- Published Online: 1 NOV 2006
- Published Print: OCT 2006
The mammalian brain contains a myriad of interconnected regions. An examination of the complex circuitry of these areas requires sensitive neuroanatomical tract tracing techniques. The anterograde tracers, Phaseolus vulgaris leucoagglutinin (PHA-L) and biotinylated dextran amines (BDA) are powerful tools that can be used to label fiber tracts that project from one particular brain region. When injected iontophoretically, PHA-L and BDA are readily taken up by neurons and transported anterogradely along their axonal tracts. Combined with immunocytochemistry for neurotransmitters, neuropeptides, and receptors, tract tracing methods may be used to elucidate the phenotype of synapses that form the microcircuitry of specific neural systems.
- Phaseolus vulgaris leucoagglutinin;
- dextran amines;
- light microscopy;
- electron microscopy;
- neuroanatomical connectivity