Unit

UNIT 1.17 Retrograde Axonal Tracing with Fluorescent Markers

  1. Brett R. Schofield

Published Online: 1 APR 2008

DOI: 10.1002/0471142301.ns0117s43

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Schofield, B. R. 2008. Retrograde Axonal Tracing with Fluorescent Markers. Current Protocols in Neuroscience. 43:1.17:1.17.1–1.17.24.

Author Information

  1. Northeastern Ohio Universities College of Medicine, Rootstown, Ohio

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008

Abstract

The growth of fluorescence imaging technology and the development of sensitive fluorescent retrograde tracers has provided many new approaches for analyzing neuronal circuits. Fluorescent markers provide unparalleled opportunity for combining axonal tract tracing with techniques such as immunohistochemistry or physiological recording. This unit describes the use of six different fluorescent tracers: Fast Blue, fluorescein dextran, FluoroGold, FluoroRuby, red beads, and green beads. Guidance is provided on how to choose a tracer for a particular experiment, and three methods are described for injecting the tracers, including pressure injection through a microsyringe or a micropipet, and iontophoretic injection through a micropipet. Criteria for selecting the most appropriate method are discussed. The protocols provide the information necessary to take advantage of the numerous fluorescent tracers that are available and to apply them to a wide variety of scientific questions. Curr. Protoc. Neurosci. 43:1.17.1-1.17.24. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • neuroanatomy;
  • tract-tracing;
  • axonal transport;
  • circuit