Unit

UNIT 1.20 Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice

  1. Julie A. Harris,
  2. Seung Wook Oh,
  3. Hongkui Zeng

Published Online: 1 APR 2012

DOI: 10.1002/0471142301.ns0120s59

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Harris, J. A., Wook Oh, S. and Zeng, H. 2012. Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice. Current Protocols in Neuroscience. 59:1.20:1.20.1–1.20.18.

Author Information

  1. Allen Institute for Brain Science, Seattle, Washington

Publication History

  1. Published Online: 1 APR 2012
  2. Published Print: APR 2012

Abstract

Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic-guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1-1.20.18. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • AAV;
  • rAAV;
  • viral tracer;
  • neural circuits;
  • anterograde tracer;
  • Cre-dependent virus