UNIT 1.20 Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice
Published Online: 1 APR 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Harris, J. A., Wook Oh, S. and Zeng, H. 2012. Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice. Current Protocols in Neuroscience. 59:1.20:1.20.1–1.20.18.
- Published Online: 1 APR 2012
- Published Print: APR 2012
Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic-guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1-1.20.18. © 2012 by John Wiley & Sons, Inc.
- viral tracer;
- neural circuits;
- anterograde tracer;
- Cre-dependent virus