UNIT 2.2 Basic Confocal Microscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Smith, C. L. 2001. Basic Confocal Microscopy. Current Protocols in Neuroscience. 00:2.2:2.2.1–2.2.13.
- Published Online: 1 MAY 2001
- Published Print: SEP 1997
This is not the most recent version of the article. View current version (1 JUL 2011)
Confocal microscopy produces sharp images of structures within relatively thick specimens (up to several hundred microns). It is particularly useful for examining fluorescent specimens. This overview intended to provide background and practical tips needed to get started with confocal microscopy. It begins with a description of the basis of optical sectioning, then discusses various types of confocal microscopes, and concludes with practical guidelines for sample preparation and optimizing image acquisition parameters.