UNIT 2.2 Basic Confocal Microscopy
Published Online: 1 JUL 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Smith, C. L. 2011. Basic Confocal Microscopy. Current Protocols in Neuroscience. 56:2.2:2.2.1–2.2.18.
- Published Online: 1 JUL 2011
- Published Print: JUL 2011
This unit introduces the reader to the basic principles of confocal microscopy and the design and capabilities of current confocal microscopes. The advantages and disadvantages of confocal microscopy compared to other techniques for fluorescence imaging are described. There are also practical guidelines for sample preparation and optimization of imaging parameters, as well as examples of some of the applications of confocal microscopy. Curr. Protoc. Neurosci. 56:2.2.1-2.2.18. © 2011 by John Wiley & Sons, Inc.
- confocal microscopy;
- three-dimensional reconstruction