UNIT 2.3 Imaging Nervous System Activity
Published Online: 1 OCT 2009
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Fields, R. D., Shneider, N., Mentis, G. Z. and O'Donovan, M. J. 2009. Imaging Nervous System Activity. Current Protocols in Neuroscience. 55:2.3.1–2.3.16.
- Published Online: 1 OCT 2009
- Published Print: OCT 2009
This unit describes methods for loading ion- and voltage-sensitive dyes into neurons, with a particular focus on the spinal cord as a model system. In addition, we describe the use of these dyes to visualize neural activity. Although the protocols described here concern spinal networks in culture or an intact in vitro preparation, they can be, and have been, widely used in other parts of the nervous system. Curr. Protoc. Neurosci. 49:2.3.1-2.3.14. © 2009 by John Wiley & Sons, Inc.
- ion-sensitive dyes;
- voltage-sensitive dyes;
- inverse Pericam;
- retrograde loading