UNIT 2.5 Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue
Published Online: 1 JAN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Delaney, K. R. 2010. Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue. Current Protocols in Neuroscience. 50:2.5:2.5.1–2.5.11.
- Published Online: 1 JAN 2010
- Published Print: JAN 2010
Dextran-conjugated Ca2+ indicators are retained well in neurons for many days following loading in intact or semi-intact brain tissue. Methods for loading neurons, as well as discussion of the unique properties of dextran-conjugated dyes which need to be considered for their use, are presented. Curr. Protoc. Neurosci. 50:2.5.1-2.5.11. © 2010 by John Wiley & Sons, Inc.
- calcium imaging;
- presynaptic terminals;
- in vivo;
- en bloc;