Unit

UNIT 2.5 Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue

  1. Kerry R. Delaney

Published Online: 1 JAN 2010

DOI: 10.1002/0471142301.ns0205s50

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Delaney, K. R. 2010. Loading Neurons with Dextran-Conjugated Calcium Indicators in Intact Nervous Tissue. Current Protocols in Neuroscience. 50:2.5:2.5.1–2.5.11.

Author Information

  1. University of Victoria, Victoria, British Columbia, Canada

Publication History

  1. Published Online: 1 JAN 2010
  2. Published Print: JAN 2010

Abstract

Dextran-conjugated Ca2+ indicators are retained well in neurons for many days following loading in intact or semi-intact brain tissue. Methods for loading neurons, as well as discussion of the unique properties of dextran-conjugated dyes which need to be considered for their use, are presented. Curr. Protoc. Neurosci. 50:2.5.1-2.5.11. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • calcium imaging;
  • dendrites;
  • presynaptic terminals;
  • in vivo;
  • en bloc;
  • electroporation