Unit

UNIT 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function

  1. Michael A. Cousin

Published Online: 1 APR 2008

DOI: 10.1002/0471142301.ns0206s43

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Cousin, M. A. 2008. Use of FM1-43 and Other Derivatives to Investigate Neuronal Function. Current Protocols in Neuroscience. 43:2.6:2.6.1–2.6.12.

Author Information

  1. University of Edinburgh, Edinburgh, Scotland

Publication History

  1. Published Online: 1 APR 2008
  2. Published Print: APR 2008

Abstract

The fluorescent dye FM1-43 and its derivatives can be used to monitor the physiology of synaptic vesicle turnover in central nerve terminals. They do so by their ability to reversibly partition into membranes, a process that results in a huge increase in fluorescence in comparison to their quantum yield in solution. This unit provides protocols for quantifying total synaptic vesicle turnover, the kinetics and extent of synaptic vesicle exocytosis, and the kinetics and mode of synaptic vesicle endocytosis. Descriptions of other ways these protocols have been used to derive information about the life cycle of the synaptic vesicle are also provided. Curr. Protoc. Neurosci. 43:2.6.1-2.6.12. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • FM1-43;
  • synaptic vesicle;
  • exocytosis;
  • endocytosis;
  • fluorescence;
  • FM2-10;
  • FM4-64