UNIT 2.6 Use of FM1-43 and Other Derivatives to Investigate Neuronal Function
Published Online: 1 APR 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Cousin, M. A. 2008. Use of FM1-43 and Other Derivatives to Investigate Neuronal Function. Current Protocols in Neuroscience. 43:2.6:2.6.1–2.6.12.
- Published Online: 1 APR 2008
- Published Print: APR 2008
The fluorescent dye FM1-43 and its derivatives can be used to monitor the physiology of synaptic vesicle turnover in central nerve terminals. They do so by their ability to reversibly partition into membranes, a process that results in a huge increase in fluorescence in comparison to their quantum yield in solution. This unit provides protocols for quantifying total synaptic vesicle turnover, the kinetics and extent of synaptic vesicle exocytosis, and the kinetics and mode of synaptic vesicle endocytosis. Descriptions of other ways these protocols have been used to derive information about the life cycle of the synaptic vesicle are also provided. Curr. Protoc. Neurosci. 43:2.6.1-2.6.12. © 2008 by John Wiley & Sons, Inc.
- synaptic vesicle;