Unit

UNIT 2.10 Whole-Mount Immunohistochemistry of the Brain

  1. Se-Hoon Kim1,
  2. Priscilla Che2,
  3. Seung-Hyuk Chung2,
  4. Debora Doorn2,
  5. Monica Hoy2,
  6. Matt Larouche2,
  7. Hassan Marzban2,
  8. Justyna Sarna2,
  9. Sepehr Zahedi2,
  10. Richard Hawkes2

Published Online: 1 AUG 2006

DOI: 10.1002/0471142301.ns0210s36

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Kim, S.-H., Che, P., Chung, S.-H., Doorn, D., Hoy, M., Larouche, M., Marzban, H., Sarna, J., Zahedi, S. and Hawkes, R. 2006. Whole-Mount Immunohistochemistry of the Brain. Current Protocols in Neuroscience. 36:2.10:2.10.1–2.10.9.

Author Information

  1. 1

    Konyang University, Chungnam, South Korea

  2. 2

    The University of Calgary, Alberta, Canada

Publication History

  1. Published Online: 1 AUG 2006
  2. Published Print: JUL 2006

Abstract

The gross anatomical distribution of an antigen is typically mapped using a combination of serial sectioning, immunocytochemistry, and three-dimensional reconstruction. This is a tedious and time-consuming procedure, which introduces an array of potential alignment and differential shrinkage errors and requires considerable experience and specialized equipment. In particular, it is unsuited for routine screening applications. To circumvent these problems, this unit presents a routine whole-mount immunocytochemistry protocol that can be used to map many antigenic distributions in the developing and adult brain. The technique can also be easily adapted to detect anterograde and retrograde transport tracers.

Keywords:

  • cerebellum;
  • development;
  • pattern formation;
  • antigen mapping;
  • anterograde transport;
  • retrograde transport