UNIT 2.14 High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy
Published Online: 1 OCT 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Takahashi, N., Oba, S., Yukinawa, N., Ujita, S., Mizunuma, M., Matsuki, N., Ishii, S. and Ikegaya, Y. 2011. High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy. Current Protocols in Neuroscience. 57:2.14:2.14.1–2.14.10.
- Published Online: 1 OCT 2011
- Published Print: OCT 2011
Conventional confocal and two-photon microscopy scan the field of view sequentially with single-point laser illumination. This raster-scanning method constrains video speeds to tens of frames per second, which are too slow to capture the temporal patterns of fast electrical events initiated by neurons. Nipkow-type spinning-disk confocal microscopy resolves this problem by the use of multiple laser beams. We describe experimental procedures for functional multineuron calcium imaging (fMCI) based on Nipkow-disk confocal microscopy, which enables us to monitor the activities of hundreds of neurons en masse at a cellular resolution at up to 2000 fps. Curr. Protoc. Neurosci. 57:2.14.1-2.14.10. © 2011 by John Wiley & Sons, Inc.