Unit

UNIT 2.17 Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F-actin

  1. Lori R. Hardy

Published Online: 1 OCT 2012

DOI: 10.1002/0471142301.ns0217s61

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Hardy, L. R. 2012. Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F-actin. Current Protocols in Neuroscience. 61:2.17:2.17.1–2.17.12.

Author Information

  1. Department of Basic Sciences, Philadelphia College of Osteopathic Medicine, Suwanee, Georgia

Publication History

  1. Published Online: 1 OCT 2012

Abstract

In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility. Curr. Protoc. Neurosci. 61:2.17.1-2.17.12. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • FRAP;
  • actin;
  • protein mobility;
  • GFP