UNIT 3.1 Culture of Neuroepithelial Stem Cells

  1. Thomas Hazel,
  2. Thomas Müller

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0301s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Hazel, T. and Müller, T. 2001. Culture of Neuroepithelial Stem Cells. Current Protocols in Neuroscience. 00:3.1:3.1.1–3.1.6.

Author Information

  1. National Institute of Neurological Disorders and Stroke, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997


In vitro culture of multipotent neural precursors allows researchers to study mechanisms regulating such processes as proliferation and lineage commitment within the developing central nervous system (CNS). The protocols presented in this unit describe the isolation and maintenance of stem cells from both fetal and adult rodent neural tissue. A procedure for treating tissue culture dishes with poly-l-ornithine and fibronectin, which is necessary before the dishes are used for neuroepithelial stem cell culture, is also included.