UNIT 3.1 Culture of Neuroepithelial Stem Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Hazel, T. and Müller, T. 2001. Culture of Neuroepithelial Stem Cells. Current Protocols in Neuroscience. 00:3.1:3.1.1–3.1.6.
- Published Online: 1 MAY 2001
- Published Print: SEP 1997
In vitro culture of multipotent neural precursors allows researchers to study mechanisms regulating such processes as proliferation and lineage commitment within the developing central nervous system (CNS). The protocols presented in this unit describe the isolation and maintenance of stem cells from both fetal and adult rodent neural tissue. A procedure for treating tissue culture dishes with poly-l-ornithine and fibronectin, which is necessary before the dishes are used for neuroepithelial stem cell culture, is also included.