UNIT 3.2 Long-Term Culture of Hippocampal Neurons
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Vicario-Abejón, C. 2004. Long-Term Culture of Hippocampal Neurons. Current Protocols in Neuroscience. 26:3.2:3.2.1–3.2.13.
- Published Online: 1 MAY 2004
- Published Print: JAN 2004
In culture, hippocampal cells can develop to express neuronal antigens and acquire mature neuronal morphologies, including axons, complex dendritic trees, and synapses that are electrophysiologically active. This system is suitable for studying neuronal differentiation and other events, such as synaptogenesis. It is also a valuable model for investigating synaptic plasticity and exploring the mechanisms of neuronal degeneration. This unit provides a protocol for culturing neurons prepared from embryonic (E-18) rat or mouse hippocampus, but could also be used to grow neurons from embryonic cortex, olfactory bulb, striatum, or spinal cord. A second method is included for preparing neuronal cultures from embryos with different genotypes, such as those from transgenic mice. Also described is the preparation of polyornithine- and fibronectin-coated coverslips, which are highly adhesive and promote neurite outgrowth, for use in the culture protocols.