Unit

UNIT 3.6 Differentiation of Embryonic Stem Cells

  1. Ahmed Mansouri1,3,5,
  2. Hidefumi Fukumitsu2,
  3. Jan Schindehuette3,
  4. Kerstin Krieglstein4,5

Published Online: 1 APR 2009

DOI: 10.1002/0471142301.ns0306s47

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Mansouri, A., Fukumitsu, H., Schindehuette, J. and Krieglstein, K. 2009. Differentiation of Embryonic Stem Cells. Current Protocols in Neuroscience. 47:3.6:3.6.1–3.6.21.

Author Information

  1. 1

    Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany

  2. 2

    Gifu Pharmaceutical University, Gifu, Japan

  3. 3

    University of Göttingen, Department of Clinical Neurophysiology, Göttingen, Germany

  4. 4

    University of Göttingen, Institute of Neuroanatomy, Göttingen, Germany

  5. 5

    DFG Center for Molecular Physiology of the Brain, Göttingen, Germany

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009

Abstract

Mouse embryonic stem (ES) cells are derived from mouse blastocyst and are able to generate all embryonic tissues in vitro. This propensity of ES cells has acquired considerable attention in recent years due to the promising potential for future cell replacement–based therapies. Therefore, it is of fundamental interest to establish protocols that allow the differentiation of ES cells into specific cell types. In recent years, several such differentiation procedures have been described for mouse and human embryonic stem cells. This unit describes a simple procedure that promotes the neuronal differentiation of mouse embryonic stem cells and yields a high proportion of midbrain dopaminergic neurons. Furthermore, this procedure permits the isolation of neural stem cell lines from mouse ES cells. Curr. Protoc. Neurosci. 47:3.6.1-3.6.21. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • embryonic stem cells;
  • dopaminergic neurons;
  • SDIA;
  • neural stem cells