Unit

UNIT 3.7 Immortalizing Central Nervous System Cells

  1. Scott R. Whittemore

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0307s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Whittemore, S. R. 2001. Immortalizing Central Nervous System Cells. Current Protocols in Neuroscience. 00:3.7:3.7.1–3.7.17.

Author Information

  1. University of Miami School of Medicine, Miami, Florida

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997

Abstract

This unit presents methods for isolating clonal, neural-derived cell lines. One approach for isolating such neural cell lines involves a replication-deficient retrovirus encoding a specific oncogene and a selectable marker which are used to infect dissociated CNS cells dissected at a developmental stage at which the cell population of interest has not undergone its terminal mitotic division. Also presented is a method for cloning by limiting dilution, which may be necessary to obtain a pure population of cells. Following growth under appropriate selection conditions, clones are isolated and tested for their ability to differentiate with the desired phenotypic properties. A method is also provided for coating tissue culture dishes, which is necessary for successful culture of CNS neurons.