UNIT 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR
Published Online: 1 JUL 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Yung, Y. C., Kennedy, G. and Chun, J. 2009. Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR. Current Protocols in Neuroscience. 48:3.8:3.8.1–3.8.24.
- Published Online: 1 JUL 2009
- Published Print: JUL 2009
Programmed cell death is a fundamental process for the development and somatic maintenance of organisms. This unit describes methods for visualizing both dying cells in situ and for detection of nucleosomal ladders. A description of various current detection strategies is provided, as well as support protocols for preparing positive and negative controls and for preparing genomic DNA. Curr. Protoc. Neurosci. 48:3.8.1-3.8.24. © 2009 by John Wiley & Sons, Inc.
- cell death;
- nucleosomal ladder