Unit

UNIT 3.8 Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR

  1. Yun C. Yung1,2,
  2. Grace Kennedy1,
  3. Jerold Chun1

Published Online: 1 JUL 2009

DOI: 10.1002/0471142301.ns0308s48

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Yung, Y. C., Kennedy, G. and Chun, J. 2009. Identification of Neural Programmed Cell Death Through the Detection of DNA Fragmentation In Situ and by PCR. Current Protocols in Neuroscience. 48:3.8:3.8.1–3.8.24.

Author Information

  1. 1

    Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, La Jolla, California

  2. 2

    University of California, San Diego School of Medicine, San Diego, California

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009

Abstract

Programmed cell death is a fundamental process for the development and somatic maintenance of organisms. This unit describes methods for visualizing both dying cells in situ and for detection of nucleosomal ladders. A description of various current detection strategies is provided, as well as support protocols for preparing positive and negative controls and for preparing genomic DNA. Curr. Protoc. Neurosci. 48:3.8.1-3.8.24. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • cell death;
  • apoptosis;
  • ISEL;
  • TUNEL;
  • nucleosomal ladder