UNIT 3.16 Gene Expression in Transgenic Mice Using Neural Promoters
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Okabe, S. 2001. Gene Expression in Transgenic Mice Using Neural Promoters. Current Protocols in Neuroscience. 7:3.16:3.16.1–3.16.19.
- Published Online: 1 MAY 2001
- Published Print: MAY 1999
In the first part of this unit, major considerations for the analysis of neural promoters in transgenic mice are discussed. Detailed protocols on the production of transgenic mice are not described in this unit. Advantages and disadvantages of the transgenic approach for analysis of neural cis-acting elements are also presented. The concept of transient transgenic mice is then introduced; this method compensates for some disadvantages associated with the conventional transgenic approach. Finally, major factors influencing the efficiency of transgenic mouse production are discussed. The second part of the unit presents detailed information on a variety of neural-specific cis-acting elements that have been characterized by a transgenic approach. This information is useful both as a guide for carrying out the analysis of cis-acting elements and as a reference for selection of promoter/enhancer elements for designing an appropriate transgenic construct.