UNIT 3.17 Isolation and Purification of Primary Schwann Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Weinstein, D. E. and Wu, R. 2001. Isolation and Purification of Primary Schwann Cells. Current Protocols in Neuroscience. 8:3.17:3.17.1–3.17.9.
- Published Online: 1 MAY 2001
- Published Print: AUG 1999
Schwann cells are the glial cells of the peripheral nervous system (PNS) which both myelinate and provide trophic support for their associated axons. Their functions are critical for proper development, homeostasis, and regeneration of the PNS. Schwann cells can be isolated and expanded in culture. Such a culture can be used as a source of myelinating glia in culture or as a source of Schwann cells for various cell and molecular biological experiments. The proliferative ability of these cells is exploited in the isolation procedures presented here.