UNIT 3.17 Isolation and Purification of Primary Schwann Cells

  1. David E. Weinstein,
  2. Rina Wu

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0317s08

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Weinstein, D. E. and Wu, R. 2001. Isolation and Purification of Primary Schwann Cells. Current Protocols in Neuroscience. 8:3.17:3.17.1–3.17.9.

Author Information

  1. Albert Einstein College Of Medicine, Bronx, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1999


Schwann cells are the glial cells of the peripheral nervous system (PNS) which both myelinate and provide trophic support for their associated axons. Their functions are critical for proper development, homeostasis, and regeneration of the PNS. Schwann cells can be isolated and expanded in culture. Such a culture can be used as a source of myelinating glia in culture or as a source of Schwann cells for various cell and molecular biological experiments. The proliferative ability of these cells is exploited in the isolation procedures presented here.