UNIT 3.23 In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
Published Online: 1 JUL 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Ito, K. and Fujita, K. 2011. In Vitro Analysis of Mouse Mesencephalic Neural Crest Development. Current Protocols in Neuroscience. 56:3.23:3.23.1–3.23.8.
- Published Online: 1 JUL 2011
- Published Print: JUL 2011
The neural crest is a unique structure in vertebrates. Neural crest cells play important roles in the formation of organs that characterize the vertebrate body plan. In this unit, we describe a primary culture method for mouse mesencephalic neural crest cells. The neural crest cells cultured by this method actively proliferate and differentiate into various cell types that originate from cranial neural crest cells, such as chondrocytes, neurons, and glia. Therefore, this primary culture method is useful for analyzing the development of mouse mesencephalic neural crest cells. Curr. Protoc. Neurosci. 56:3.23.1-3.23.8. © 2011 by John Wiley & Sons, Inc.
- neural crest cells;
- primary culture