Unit

UNIT 3.23 In Vitro Analysis of Mouse Mesencephalic Neural Crest Development

  1. Kazuo Ito,
  2. Kyohei Fujita

Published Online: 1 JUL 2011

DOI: 10.1002/0471142301.ns0323s56

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Ito, K. and Fujita, K. 2011. In Vitro Analysis of Mouse Mesencephalic Neural Crest Development. Current Protocols in Neuroscience. 56:3.23:3.23.1–3.23.8.

Author Information

  1. Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Japan

Publication History

  1. Published Online: 1 JUL 2011
  2. Published Print: JUL 2011

Abstract

The neural crest is a unique structure in vertebrates. Neural crest cells play important roles in the formation of organs that characterize the vertebrate body plan. In this unit, we describe a primary culture method for mouse mesencephalic neural crest cells. The neural crest cells cultured by this method actively proliferate and differentiate into various cell types that originate from cranial neural crest cells, such as chondrocytes, neurons, and glia. Therefore, this primary culture method is useful for analyzing the development of mouse mesencephalic neural crest cells. Curr. Protoc. Neurosci. 56:3.23.1-3.23.8. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • mesencephalon;
  • neural crest cells;
  • primary culture