Unit

UNIT 3.24 Culture of Mouse Olfactory Sensory Neurons

  1. Qizhi Gong

Published Online: 1 JAN 2012

DOI: 10.1002/0471142301.ns0324s58

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Gong, Q. 2012. Culture of Mouse Olfactory Sensory Neurons. Current Protocols in Neuroscience. 58:3.24:3.24.1–3.24.14.

Author Information

  1. Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, California

Publication History

  1. Published Online: 1 JAN 2012
  2. Published Print: JAN 2012

Abstract

Olfactory sensory neurons, located in the nasal epithelium, detect and transmit odorant information to the central nervous system. This requires that these neurons form specific neuronal connections within the olfactory bulb and express receptors and signaling molecules specific for these functions. This protocol describes a primary olfactory sensory neuron culture technique that allows in vitro investigation of olfactory sensory neuron differentiation, axon outgrowth, odorant receptor expression, and function. Olfactory epithelium is obtained from the nasal cavity and is enzymatically treated to reduce stroma tissue. Dissociated olfactory sensory neurons are cultured directly on a layer of cortical astrocytes to support their survival. Using this method, cultured olfactory sensory neurons maintain their bipolar morphology and express odorant signal transduction molecules, which are specific for olfactory sensory neurons. Curr. Protoc. Neurosci. 58:3.24.1-3.24.14. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • olfactory epithelium;
  • bipolar;
  • astrocytes;
  • lentivirus;
  • odorant receptor