Unit

UNIT 4.5 Overview of Neural Gene Expression

  1. Rachael L. Neve1,
  2. Kim A. Neve2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0405s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Neve, R. L. and Neve, K. A. 2001. Overview of Neural Gene Expression. Current Protocols in Neuroscience. 00:4.5:4.5.1–4.5.4.

Author Information

  1. 1

    Harvard Medical School and McLean Hospital, Belmont, Massachusetts

  2. 2

    Oregon Health Sciences University and Veterans Affairs Medical Center, Portland, Oregon

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997

Abstract

This overview describes issues that must be considered before attempting to express neural cDNAs in mammalian cells, including the choice of expression vector and cell type. Considerations for introducing recombinant vectors into cells are discussed along with a comparison of achieving stable or transient expression. Finally, the appropriate promoter is crucial and must be chosen to fit the design of the expression system.