UNIT 4.5 Overview of Neural Gene Expression
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Neve, R. L. and Neve, K. A. 2001. Overview of Neural Gene Expression. Current Protocols in Neuroscience. 00:4.5:4.5.1–4.5.4.
- Published Online: 1 MAY 2001
- Published Print: SEP 1997
This overview describes issues that must be considered before attempting to express neural cDNAs in mammalian cells, including the choice of expression vector and cell type. Considerations for introducing recombinant vectors into cells are discussed along with a comparison of achieving stable or transient expression. Finally, the appropriate promoter is crucial and must be chosen to fit the design of the expression system.