UNIT 4.9 Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Strader, C. D. 2001. Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships. Current Protocols in Neuroscience. 3:4.9:4.9.1–4.9.5.
- Published Online: 1 MAY 2001
- Published Print: MAY 1998
Several mutagenesis strategies have been used to allow the identification of specific receptor-ligand interactions to support the docking of ligands within various receptor models. The strengths and limitations of each strategy are discussed in this overview. Large-scale mapping is described using deletion and chimeric receptors. Fine tuning with single residue replacements is also covered along with two-dimensional approaches.