Unit

UNIT 4.9 Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships

  1. Catherine D. Strader

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0409s03

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Strader, C. D. 2001. Mutagenesis Approaches for Elucidation of Protein Structure-Function Relationships. Current Protocols in Neuroscience. 3:4.9:4.9.1–4.9.5.

Author Information

  1. Schering-Plough Research Institute, Kenilworth, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1998

Abstract

Several mutagenesis strategies have been used to allow the identification of specific receptor-ligand interactions to support the docking of ligands within various receptor models. The strengths and limitations of each strategy are discussed in this overview. Large-scale mapping is described using deletion and chimeric receptors. Fine tuning with single residue replacements is also covered along with two-dimensional approaches.