UNIT 4.13 Generation of High-Titer Defective HSV-1 Vectors
Published Online: 1 JAN 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Neve, R. L. and Lim, F. 2013. Generation of High-Titer Defective HSV-1 Vectors. Current Protocols in Neuroscience. 62:4.13.1–4.13.15.
- Published Online: 1 JAN 2013
Basic Protocol 1 describes the generation of helper virus stocks. Preparation of recombinant amplicon vector particles by transfection of amplicon and superinfection of helper virus into cells, and harvesting of packaged particles, is delineated in Basic Protocol 3. Thorough characterization of each amplicon viral vector stock involves measuring (1) the helper virus plaque-forming units per ml (pfu/ml) on 2-2 cells and (2) the amplicon stock infectious units per ml (iu/ml) on PC12 cells. The Support Protocols detail methods for determining titers of helper virus by plaque assay, and of amplicon stocks by vector assay. Curr. Protoc. Neurosci. 62:4.13.1-4.13.15. © 2013 by John Wiley & Sons, Inc.
Keywords: HSV-1; amplicon; defective vector