Unit

UNIT 4.17 Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration

  1. Steven J. Gray,
  2. Vivian W. Choi,
  3. Aravind Asokan,
  4. Rebecca A. Haberman,
  5. Thomas J. McCown,
  6. Richard Jude Samulski

Published Online: 1 OCT 2011

DOI: 10.1002/0471142301.ns0417s57

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Gray, S. J., Choi, V. W., Asokan, A., Haberman, R. A., McCown, T. J. and Samulski, R. J. 2011. Production of Recombinant Adeno-Associated Viral Vectors and Use in In Vitro and In Vivo Administration. Current Protocols in Neuroscience. 57:4.17:4.17.1–4.17.30.

Author Information

  1. University of North Carolina at Chapel Hill, School of Medicine, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011

Abstract

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP. Curr. Protoc. Neurosci. 57:4.17.1-4.17.30. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • gene therapy;
  • viral vectors;
  • adeno-associated virus;
  • purification;
  • serotypes;
  • transfection;
  • brain;
  • spinal cord;
  • transduction;
  • gene delivery