UNIT 4.18 Overview of the Baculovirus Expression System
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Murphy, C. I. and Piwnica-Worms, H. 2001. Overview of the Baculovirus Expression System. Current Protocols in Neuroscience. 10:4.18:4.18.1–4.18.4.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.