Unit

UNIT 4.18 Overview of the Baculovirus Expression System

  1. Cheryl Isaac Murphy1,
  2. Helen Piwnica-Worms2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0418s10

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Murphy, C. I. and Piwnica-Worms, H. 2001. Overview of the Baculovirus Expression System. Current Protocols in Neuroscience. 10:4.18:4.18.1–4.18.4.

Author Information

  1. 1

    Cambridge Biotech Corporation, Worcester, Massachusetts

  2. 2

    Washington University School of Medicine, St.Louis, Missouri

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.