Unit

UNIT 4.21 Production and Titration of Lentiviral Vectors

  1. Isabelle Barde1,
  2. Patrick Salmon2,
  3. Didier Trono1

Published Online: 1 OCT 2010

DOI: 10.1002/0471142301.ns0421s53

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Barde, I., Salmon, P. and Trono, D. 2010. Production and Titration of Lentiviral Vectors. Current Protocols in Neuroscience. 53:4.21:4.21.1–4.21.23.

Author Information

  1. 1

    School of Life Sciences, École Polytechnique Fédérale de Lausanne and “Frontiers in Genetics,” National Center of Competence in Research, Lausanne, Switzerland

  2. 2

    Department of Neuroscience. Faculty of Medicine, University of Geneva, Geneva, Switzerland

Publication History

  1. Published Online: 1 OCT 2010
  2. Published Print: OCT 2010

Abstract

Lentiviral vectors have emerged over the last decade as powerful, reliable, and safe tools for stable gene transfer in a wide variety of mammalian cells. Unlike other vectors derived from oncoretroviruses, they allow for stable gene delivery into most nondividing primary cells, including neurons. This is why lentivectors (LVs) are becoming the most useful and promising tools in the field of neuroscience, not only for research, but also for future gene and cell therapy approaches. LVs derived from HIV-1 have gradually evolved to display many desirable features aimed at increasing both their safety and their versatility. These latest designs are reviewed in this unit. This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories. Curr. Protoc. Neurosci. 53:4.21.1-4.21.23. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • lentivirus vector;
  • lentivector;
  • gene transfer;
  • molecular biology;
  • gene expression;
  • protein expression;
  • neuroscience;
  • molecular neurobiology;
  • RNA;
  • viral RNAs