UNIT 4.25 Overview of Nucleic Acid Arrays

  1. Joseph DeRisi

Published Online: 1 NOV 2001

DOI: 10.1002/0471142301.ns0425s16

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

DeRisi, J. 2001. Overview of Nucleic Acid Arrays. Current Protocols in Neuroscience. 16:4.25:4.25.1–4.25.6.

Author Information

  1. University of California, San Francisco, San Francisco, California

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: AUG 2001


Nucleic acid array technology refers to the use and fabrication of arrays containing thousands of nucleic acid samples bound to solid substrates such as glass microscope slides or silicon wafers. Because the physical area occupied by each sample is usually 50 to 200 micrometers in diameter, it is possible to assay nucleic acid samples representing entire genomes, ranging in size from 3,000 to 32,000 genes, on a single slide. Microarrays are useful for analyzing gene expression patterns, genotyping and genetic mapping, comparative genomic hybridization, polysome analysis, and DNA-protein interactions. This overview describes the technology and applications, and provides valuable web site listings for obtaining additional information.