Unit

UNIT 4.28 Gene Expression Analysis of Neural Cells and Tissues Using DNA Microarrays

  1. Stanislav L. Karsten,
  2. Lili C. Kudo,
  3. Daniel H. Geschwind

Published Online: 1 OCT 2008

DOI: 10.1002/0471142301.ns0428s45

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Karsten, S. L., Kudo, L. C. and Geschwind, D. H. 2008. Gene Expression Analysis of Neural Cells and Tissues Using DNA Microarrays. Current Protocols in Neuroscience. 45:4.28:4.28.1–4.28.38.

Author Information

  1. David Geffen School of Medicine at UCLA, Los Angeles, California

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

Abstract

DNA microarrays pose specific challenges to those studying the central and peripheral nervous systems. Probably the most important involve difficulty in obtaining appropriate tissue for study, as well as the problems posed by cellular heterogeneity. This unit describes advances in the available technologies and provides protocols for cDNA microarray hybridization, including the use of PCR amplicons. Protocols are also provided for the two major methods for limiting cellular heterogeneity by study of RNA from single cell populations in high-throughput microarray studies, laser capture microdissection (LCM), and automated fluorescent cell sorting (FACS-array). Curr. Protoc. Neurosci. 45:4.28.1-4.28.38. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • gene expression;
  • flow sorting;
  • single cell analysis;
  • laser capture;
  • microarray analysis