Unit

UNIT 4.34 Transfection of Mouse Cochlear Explants by Electroporation

  1. Elizabeth C. Driver,
  2. Matthew W. Kelley

Published Online: 1 APR 2010

DOI: 10.1002/0471142301.ns0434s51

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Driver, E. C. and Kelley, M. W. 2010. Transfection of Mouse Cochlear Explants by Electroporation. Current Protocols in Neuroscience. 51:4.34:4.34.1–4.34.10.

Author Information

  1. National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 APR 2010
  2. Published Print: APR 2010

Abstract

The sensory epithelium of the mammalian inner ear, also referred to as the organ of Corti, is a remarkable structure comprised of highly ordered rows of mechanosensory hair cells and non-sensory supporting cells located within the coiled cochlea. This unit describes an in vitro explant culture technique that can be coupled with gene transfer via electroporation to study the effects of altering gene expression during development of the organ of Corti. While the protocol is largely focused on embryonic cochlea, the same basic protocol can be used on cochleae from mice as old as P5. Curr. Protoc. Neurosci. 51:4.34.1-4.34.10. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • hair cell;
  • hearing;
  • cell fate;
  • mechanosensory cells;
  • organ of Corti