Unit

UNIT 5.17 Analysis of RNA by Northern and Slot-Blot Hybridization

  1. Terry Brown1,
  2. Karol Mackey (probe removal)2

Published Online: 1 AUG 2001

DOI: 10.1002/0471142301.ns0517s15

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Brown, T. and Mackey, K. 2001. Analysis of RNA by Northern and Slot-Blot Hybridization. Current Protocols in Neuroscience. 15:5.17:5.17.1–5.17.17.

Author Information

  1. 1

    University of Manchester Institute of Science and Technology, Manchester, United Kingdom

  2. 2

    Molecular Research, Cincinnati, Ohio

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: MAY 2001

Abstract

Specific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those originally developed for DNA. Fractionated RNA is transferred from an agarose gel to a membrane support (northern blotting), while unfractionated RNA is immobilized by slot or dot blotting. The resulting blots are studied by hybridization analysis with labeled DNA or RNA probes. Included in this unit are detailed procedures for RNA denaturation, blotting and hybridization. Also described is a method for stripped hybridization probes from blots so the blots can be re-hybridized with a different probe.