UNIT 5.17 Analysis of RNA by Northern and Slot-Blot Hybridization
Published Online: 1 AUG 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Brown, T. and Mackey, K. 2001. Analysis of RNA by Northern and Slot-Blot Hybridization. Current Protocols in Neuroscience. 15:5.17:5.17.1–5.17.17.
- Published Online: 1 AUG 2001
- Published Print: MAY 2001
Specific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those originally developed for DNA. Fractionated RNA is transferred from an agarose gel to a membrane support (northern blotting), while unfractionated RNA is immobilized by slot or dot blotting. The resulting blots are studied by hybridization analysis with labeled DNA or RNA probes. Included in this unit are detailed procedures for RNA denaturation, blotting and hybridization. Also described is a method for stripped hybridization probes from blots so the blots can be re-hybridized with a different probe.