Unit

UNIT 5.20 An Overview on the Generation of BAC Transgenic Mice for Neuroscience Research

  1. X. William Yang1,
  2. Shiaoching Gong2

Published Online: 1 MAY 2005

DOI: 10.1002/0471142301.ns0520s31

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Yang, X. W. and Gong, S. 2005. An Overview on the Generation of BAC Transgenic Mice for Neuroscience Research. Current Protocols in Neuroscience. 31:5.20:5.20.1–5.20.11.

Author Information

  1. 1

    Department of Psychiatry and Biobehavioral Sciences, Center for Neurobehavioral Genetics, Neuropsychiatric Institute, David Geffen School of Medicine at UCLA, Los Angeles, California

  2. 2

    Rockefeller University, New York, New York

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005

Abstract

This unit provides a comprehensive overview on the generation of transgenic mice using bacterial artificial chromosomes (BACs), and the application of BAC transgenic mice in neuroscience research. In the first section, advantages of the BAC transgenic approach compared to the conventional transgenic approach are summarized. In the second section, important considerations in designing BAC transgenic constructs are outlined. Four commonly used BAC transgenic construct designs are also outlined. Concepts of modifying BACs by homologous recombination in E. coli to introduce a variety of mutations into BACs, and important steps to characterize a modified BAC prior to the generation of transgenic mice are also presented. In the final section, some of the important applications of BAC transgenic mice in neuroscience research, including studying gene expression, gene function, mapping neuronal circuitry, and modeling human diseases, are described.