Unit

UNIT 6.3 Fabrication of Patch Pipets

  1. J.L. Rae1,
  2. R.A. Levis2

Published Online: 1 MAY 2004

DOI: 10.1002/0471142301.ns0603s26

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Rae, J. and Levis, R. 2004. Fabrication of Patch Pipets. Current Protocols in Neuroscience. 26:6.3:6.3.1–6.3.32.

Author Information

  1. 1

    Mayo Foundation, Rochester, Minnesota

  2. 2

    Rush Medical College, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: JAN 2004

Abstract

Patch clamping refers to a wide range of electrophysiological measurements, all of which have in common the use of patch pipets and the formation of gigaohm seals. The purpose of this unit is to describe the fabrication of patch pipets. The aspects of the pipet geometry that are important to different applications and the different procedures that have been found to most reliably and simply achieve these results are described. Parameters for glass selection are detailed in the beginning of the unit. Pulling patch and whole-cell pipets, elastomer coating, fire polishing, pipet filling, and pipet testing in an experimental setup are highlighted. Additional support protocols describe alternative ways to optimize pipet geometry and cleaning the glass before pulling. Considerations for noise and dynamic performance are emphasized as these two requirements for single-channel and whole-cell current measurements dictate how the pipets must be fabricated.