Unit

UNIT 6.14 Amperometry and Cyclic Voltammetry with Carbon Fiber Microelectrodes at Single Cells

  1. Michelle L. Mundorf,
  2. R. Mark Wightman

Published Online: 1 MAY 2002

DOI: 10.1002/0471142301.ns0614s18

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Mundorf, M. L. and Wightman, R. M. 2002. Amperometry and Cyclic Voltammetry with Carbon Fiber Microelectrodes at Single Cells. Current Protocols in Neuroscience. 18:6.14:6.14.1–6.14.22.

Author Information

  1. The University of north carolina at Chapel Hill, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: JAN 2002

Abstract

Amperometry and cyclic voltammetry are two electrochemical techniques that enable the detection of electroactive neurotransmitters that are released from single cells. These techniques have provided the first chemical view of the events that occur during exocytosis. This unit describes the isolation of several cell types known for their exocytotic properties, the fabrication and calibration of carbon fiber microelectrodes, as well as some of the equipment and software requirements for obtaining electrochemically generated data.