Unit

UNIT 6.15 Preparation and Maintenance of Organotypic Cultures for Multi-Electrode Array Recordings

  1. Veronica C. Karpiak,
  2. Dietmar Plenz

Published Online: 1 AUG 2002

DOI: 10.1002/0471142301.ns0615s19

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Karpiak, V. C. and Plenz, D. 2002. Preparation and Maintenance of Organotypic Cultures for Multi-Electrode Array Recordings. Current Protocols in Neuroscience. 19:6.15:6.15.1–6.15.8.

Author Information

  1. Laboratory of Systems Neuroscience, NIMH NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: APR 2002

Abstract

Preparation and Maintenance of Organotypic Cultures for Multi-Electrode Array Recordings (Veronica C. Karpiak and Dietmar Plenz, National Institutes of Mental Health, NIH, Bethesda, Maryland). Recording from neuronal cultures with multi-electrode arrays (MEAs) provides a powerful tool for studying neuronal activity with many neurons simultaneously in vitro. This unit describes the detailed steps necessary for growing organotypic cultures on MEAs and the typical neuronal activity that is obtained with this methodology.