Unit

UNIT 6.17 Imaging Nervous System Activity with Voltage-Sensitive Dyes

  1. Dejan Zecevic1,
  2. Maja Djurisic2,
  3. Lawrence B. Cohen3,
  4. Srdjan Antic3,
  5. Matt Wachowiak4,
  6. Chun X. Falk4,
  7. Michal R. Zochowski5

Published Online: 1 AUG 2003

DOI: 10.1002/0471142301.ns0617s23

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Zecevic, D., Djurisic, M., Cohen, L. B., Antic, S., Wachowiak, M., Falk, C. X. and Zochowski, M. R. 2003. Imaging Nervous System Activity with Voltage-Sensitive Dyes. Current Protocols in Neuroscience. 23:6.17:6.17.1–6.17.29.

Author Information

  1. 1

    Yale University School of Medicine, New Haven, Connecticut

  2. 2

    RedShirtImaging, Fairfield, Connecticut

  3. 3

    Yale University School of Medicine, New Haven, Connecticut

  4. 4

    Warsaw School of Advanced Social Psychology, Warsaw, Poland

  5. 5

    Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: APR 2003

Abstract

Optical recording with a voltage-sensitive dye is advantageous where membrane potential must be recorded in many sites at once. This unit describes methods for making voltage-sensitive dye measurements on different preparations to study (1) how a neuron integrates its synaptic input into its action potential output by measuring membrane potential everywhere synaptic input occurs and where spikes are initiated; (2) how a nervous system generates a behavior in Aplysia abdominal ganglion; and (3) responses to sensory stimuli and generation of motor output in the vertebrate brain by simultaneous measurement of population signals from many areas. The approach is three-pronged: (1) find the dye with the largest signal-to-noise ratio; (2) reduce extraneous sources of noise; and (3) maximize the number of photons measured to reduce the relative shot noise. A discussion of optical recording methods including the choice of dyes, light sources, optics, cameras, and minimizing noise is also provided.