UNIT 6.17 Imaging Nervous System Activity with Voltage-Sensitive Dyes
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Zecevic, D., Djurisic, M., Cohen, L. B., Antic, S., Wachowiak, M., Falk, C. X. and Zochowski, M. R. 2003. Imaging Nervous System Activity with Voltage-Sensitive Dyes. Current Protocols in Neuroscience. 23:6.17:6.17.1–6.17.29.
- Published Online: 1 AUG 2003
- Published Print: APR 2003
Optical recording with a voltage-sensitive dye is advantageous where membrane potential must be recorded in many sites at once. This unit describes methods for making voltage-sensitive dye measurements on different preparations to study (1) how a neuron integrates its synaptic input into its action potential output by measuring membrane potential everywhere synaptic input occurs and where spikes are initiated; (2) how a nervous system generates a behavior in Aplysia abdominal ganglion; and (3) responses to sensory stimuli and generation of motor output in the vertebrate brain by simultaneous measurement of population signals from many areas. The approach is three-pronged: (1) find the dye with the largest signal-to-noise ratio; (2) reduce extraneous sources of noise; and (3) maximize the number of photons measured to reduce the relative shot noise. A discussion of optical recording methods including the choice of dyes, light sources, optics, cameras, and minimizing noise is also provided.