Unit

UNIT 6.19 Patch-Clamp Recording from Neuronal Dendrites

  1. Nicholas P. Poolos,
  2. Terrance D. Jones

Published Online: 1 NOV 2004

DOI: 10.1002/0471142301.ns0619s29

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Poolos, N. P. and Jones, T. D. 2004. Patch-Clamp Recording from Neuronal Dendrites. Current Protocols in Neuroscience. 29:6.19:6.19.1–6.19.13.

Author Information

  1. University of Washington, Seattle, Washington

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2004

Abstract

Pyramidal neurons of the central nervous system have extensively arborized apical dendrites that contribute importantly to the signaling properties of the neuron. Recent advances in electrophysiological techniques have allowed recording from neuronal dendrites. These techniques depend on using infrared optics to visualize dendritic processes in the unstained brain slice preparation, on pipet positioning with high resolution micromanipulators, and on stringent techniques for brain slice preparation that preserved healthy dendritic processes, even in tissue from mature animals. The procedures underlying these techniques are described in this unit.

Keywords:

  • dendrites;
  • patch-clamp recording;
  • brain slice;
  • hippocampus;
  • IR-DIC microscopy