UNIT 6.22 Whole-Cell Recording In Vivo

  1. Michael R. DeWeese

Published Online: 1 JAN 2007

DOI: 10.1002/0471142301.ns0622s38

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

R. DeWeese, M. 2007. Whole-Cell Recording In Vivo. Current Protocols in Neuroscience. 38:6.22:6.22.1–6.22.15.

Author Information

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: JAN 2007


In vivo whole-cell patch-clamp recording provides a means for measuring membrane currents and potentials from individual cells in the intact animal. Patch-clamp methods have largely been developed in vitro. This body of work has contributed enormously to the understanding of many important phenomena in excitable cells—including synaptic plasticity in the mammalian central nervous system, and the behavior of individual protein channels. In recent years, an increasing number of groups have applied whole-cell recording techniques in the intact animal. Such in vivo studies offer the tantalizing possibility of uncovering the underlying principles and mechanisms of neural interactions within the natural context of fully intact biological networks. This unit focuses on strategies for overcoming the specific technical challenges posed by in vivo whole-cell recording. A straightforward procedure is described for obtaining whole-cell records from the cortex of the anesthetized rat; this procedure has also been applied successfully to awake animals and other rodent species with minor modifications.


  • whole-cell;
  • patch clamp;
  • in vivo;
  • intracellular recording;
  • cortex;
  • neuron;
  • rat