Unit

UNIT 6.23 Electrophysiological Recordings from Neonatal Neocortical Brain Slices

  1. Michael Daw1,
  2. John Isaac2

Published Online: 1 JAN 2007

DOI: 10.1002/0471142301.ns0623s38

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Daw, M. and Isaac, J. 2007. Electrophysiological Recordings from Neonatal Neocortical Brain Slices. Current Protocols in Neuroscience. 38:6.23:6.23.1–6.23.12.

Author Information

  1. 1

    University of Bristol, Bristol, UK

  2. 2

    NINDS/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 JAN 2007
  2. Published Print: JAN 2007

Abstract

Many brain processes and the expression of many neuronal proteins are developmentally regulated; therefore, studying cellular and molecular processes in tissue from very young animals is vital to understanding the development of the brain. Working on tissue from very young animals (first postnatal week) presents a number of specific problems. This unit describes techniques to overcome these problems to produce in vitro slices from neonatal rodents and to reliably achieve high-quality patch-clamp recordings from neurons in neonatal brain slices.

Keywords:

  • patch clamp;
  • neonatal;
  • cortex;
  • thalamocortical;
  • rat;
  • mouse;
  • development