UNIT 7.2 Microdialysis in Rodents
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Zapata, A., Chefer, V. I. and Shippenberg, T. S. 2009. Microdialysis in Rodents. Current Protocols in Neuroscience. 47:7.2:7.2.1–7.2.29.
- Published Online: 1 APR 2009
- Published Print: APR 2009
Microdialysis is an in vivo sampling technique that permits the quantification of various substances (e.g., neurotransmitters, peptides, electrolytes) in blood and tissue. It is also used to infuse substances into the brain and spinal cord. This unit describes methods for the construction and stereotaxic implantation of microdialysis probes into discrete brain regions of the rat and mouse. Procedures for the conduct of conventional and quantitative microdialysis experiments in the awake and anesthetized rodent are also provided. Curr. Protoc. Neurosci. 47:7.2.1-7.2.29. © 2009 by John Wiley & Sons, Inc.
- microdialysis probe construction;
- stereotaxic surgery;
- liquid swivel;
- dialysate sample;
- concentric dialysis probe