UNIT 7.3 Microdialysis in Nonhuman Primates

  1. Richard C. Saunders,
  2. Bhaskar S. Kolachana,
  3. Daniel R. Weinberger

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0703s00

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Saunders, R. C., Kolachana, B. S. and Weinberger, D. R. 2001. Microdialysis in Nonhuman Primates. Current Protocols in Neuroscience. 00:7.3:7.3.1–7.3.20.

Author Information

  1. NIMH/Clinical Brain Disorders Branch, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997


This unit presents a technique that allows for routine repeated microdialysis experiments in the monkey using a sedated preparation or, with further minor modification, in the awake behaving animal. Protocols are provided for construction and in vitro calibration of dialysis probes, obtaining magnetic resonance imaging (MRI) scans of the monkey brain, surgically attaching a guide holder to the skull, and performing postoperative MRI scanning, in vivo dialysis sample collection, anatomical verification of the probes, and neurochemical analysis. An alternate protocol describes microdialysis in awake, behaving monkeys; this requires substantial preparatory work in training the monkeys to sit quietly in a restraining chair and/or to perform a series of behavioral tasks.