UNIT 7.4 Detection and Quantification of Neurotransmitters in Dialysates
Published Online: 1 JUL 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Zapata, A., Chefer, V. I., Shippenberg, T. S. and Denoroy, L. 2009. Detection and Quantification of Neurotransmitters in Dialysates. Current Protocols in Neuroscience. 48:7.4:7.4.1–7.4.30.
- Published Online: 1 JUL 2009
- Published Print: JUL 2009
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Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-pressure liquid chromatography electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection; capillary electrophoresis with laser-induced fluorescence detection).Curr. Protoc. Neurosci. 48:7.4.1-7.4.30. © 2009 by John Wiley & Sons, Inc.
- high pressure liquid chromatography (HPLC);
- electrochemical detection;
- capillary electrophoresis;
- laser fluorescence;
- amino acids;