Unit

UNIT 7.4 Detection and Quantification of Neurotransmitters in Dialysates

  1. Agustin Zapata1,
  2. Vladimir I. Chefer1,
  3. Toni S. Shippenberg1,
  4. Luc Denoroy2

Published Online: 1 JUL 2009

DOI: 10.1002/0471142301.ns0704s48

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Zapata, A., Chefer, V. I., Shippenberg, T. S. and Denoroy, L. 2009. Detection and Quantification of Neurotransmitters in Dialysates. Current Protocols in Neuroscience. 48:7.4:7.4.1–7.4.30.

Author Information

  1. 1

    NIH, NIDA Intramural Research Program, Baltimore, Maryland

  2. 2

    Laboratoire de Neuropharmacologie, Institut Fédératif des Neurosciences de Lyon, Lyon, France

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009

This is not the most recent version of the article. View current version (1 APR 2013)

Abstract

Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-pressure liquid chromatography electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection; capillary electrophoresis with laser-induced fluorescence detection).Curr. Protoc. Neurosci. 48:7.4.1-7.4.30. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • high pressure liquid chromatography (HPLC);
  • electrochemical detection;
  • capillary electrophoresis;
  • laser fluorescence;
  • monoamines;
  • amino acids;
  • acetylcholine