Unit

UNIT 7.14 Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor

  1. Tadeusz Malinski1,
  2. Igor Huk2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142301.ns0714s06

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Malinski, T. and Huk, I. 2001. Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor. Current Protocols in Neuroscience. 6:7.14:7.14.1–7.14.20.

Author Information

  1. 1

    Center of Biomedical Research, Oakland University, Rochester, Michigan

  2. 2

    University of Vienna, Vienna, Austria

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1999

Abstract

This unit describes the preparation and applications of porphyrinic sensors for quantitative measurement of nitric oxide (NO) in single cells and in tissues. The determination of NO is based on the electrochemical oxidation of NO on a carbon fiber electrode covered with a thin layer of a conducting polymeric metalloporphyrin catalyst, overlaid with another thin film of Nafion, a cation exchange material. The electric current generated during NO oxidation on the surface of the polymeric porphyrin is linearly proportional to the concentration of NO, so this current is used as an analytical signal which can be measured in either the amperometric or the voltammetric mode. Both methods provide a quantitative signal. This unit describes the electrochemical setup for measurement of NO in single cells and tissue. Support protocols describe porphyrin synthesis, sensor preparation, and sensor calibration.