UNIT 7.14 Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Malinski, T. and Huk, I. 2001. Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor. Current Protocols in Neuroscience. 6:7.14:7.14.1–7.14.20.
- Published Online: 1 MAY 2001
- Published Print: FEB 1999
This unit describes the preparation and applications of porphyrinic sensors for quantitative measurement of nitric oxide (NO) in single cells and in tissues. The determination of NO is based on the electrochemical oxidation of NO on a carbon fiber electrode covered with a thin layer of a conducting polymeric metalloporphyrin catalyst, overlaid with another thin film of Nafion, a cation exchange material. The electric current generated during NO oxidation on the surface of the polymeric porphyrin is linearly proportional to the concentration of NO, so this current is used as an analytical signal which can be measured in either the amperometric or the voltammetric mode. Both methods provide a quantitative signal. This unit describes the electrochemical setup for measurement of NO in single cells and tissue. Support protocols describe porphyrin synthesis, sensor preparation, and sensor calibration.