UNIT 7.19 In Vivo Measurement of Blood-Brain Barrier Permeability

  1. Kathleen M.K. Boje

Published Online: 1 AUG 2001

DOI: 10.1002/0471142301.ns0719s15

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Boje, K. M. 2001. In Vivo Measurement of Blood-Brain Barrier Permeability. Current Protocols in Neuroscience. 15:7.19:7.19.1–7.19.39.

Author Information

  1. University at Buffalo, School of Pharmacy, Buffalo, New York

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: MAY 2001


This unit describes various protocols for the in vivo quantitation of drug permeability across the rodent blood - brain barrier. Methods for the measurement of drug influx or efflux are described, and support protocols are provided for determining intravascular capillary volume and cerebral perfusion flow. An in situ perfusion technique is also provided for assessing whether transport of a test compound occurs by carrier-mediated or saturable transport.