Unit

UNIT 7.20 Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid β-Peptides

  1. Paul A. Hyslop,
  2. Mark H. Bender

Published Online: 1 MAY 2002

DOI: 10.1002/0471142301.ns0720s18

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Hyslop, P. A. and Bender, M. H. 2002. Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid β-Peptides. Current Protocols in Neuroscience. 18:7.20:7.20.1–7.20.11.

Author Information

  1. Eli Lilly and Company, Indianapolis, Indiana

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: JAN 2002

Abstract

Use of low abundance analytes in whole tissue homogenates has been realized with the development of assays in which a specific analyte is captured and detected using immunological reagents. One of the many advantages of analyte immunoassay in crude homogenates is its relative simplicity, allowing high throughput analysis of samples. In this unit, some major key determinants in sample and standard preparation and handling are described that have been shown to improve the performance and reliability of these assay systems. The ELISA assay of amyloid peptides from brain tissue is described as an example, since the protocols for this analysis exemplify many of the techniques and problems that are encountered in the development of new assays.