UNIT 9.37 Pentylenetetrazol (PTZ) Kindling Model of Epilepsy
Published Online: 1 JAN 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Neuroscience
How to Cite
Dhir, A. 2012. Pentylenetetrazol (PTZ) Kindling Model of Epilepsy. Current Protocols in Neuroscience. 58:9.37:9.37.1–9.37.12.
- Published Online: 1 JAN 2012
- Published Print: JAN 2012
This unit describes a protocol to perform chemical kindling in mice. Kindling is a chronic animal model of epilepsy that has been extensively studied to understand the process of epileptogenesis and discover novel anti-epileptic compounds. Kindling is a phenomenon where a sub-convulsive stimulus (either chemical or electrical), if applied repetitively and intermittently, will ultimately lead to the generation of full-blown convulsions. Kindling can be induced either by (1) electrical stimulation of different brain regions (electrical kindling) or (2) using various chemical agents (chemical kindling). This unit discusses in detail the methodology to execute pentylenetetrazol (PTZ; a GABAA receptor antagonist)–induced chemical kindling in mice. PTZ is administered chronically at a sub-convulsive dose for a number of days. Seizure score is calculated after each PTZ injection. The effect of test/reference compounds can be tested by administering them either prior to the initiation of kindling (pre-kindling phase) or after animals are fully kindled (post-kindling phase). Curr. Protoc. Neurosci. 58:9.37.1-9.37.12. © 2012 by John Wiley & Sons, Inc.
- chemical kindling;